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Pregled bibliografske jedinice broj: 262422

Evaluation Of Different Techniques For Hpv Typing Of Low Prevalent Types


Sabol, Ivan; Salakova, Martina; Smahelova, Jana; Milutin Gasperov, Nina; Tachezy, Ruth; Grce, Magdalena
Evaluation Of Different Techniques For Hpv Typing Of Low Prevalent Types // 23rd International Papillomavirus Conference & Clinical Workshop : Book of abstracts / Hamsikova, Eva ; Haugen, Thomas H ; Palefsky, Joel ; Smith, Elaine M ; Tachezy, Ruth ; Turek, Lubomir P ; Vonka, Vladimir (ur.).
Prag: The Institute of Hematology and Blood Transfusion, 2006. str. 370-370 (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 262422 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Evaluation Of Different Techniques For Hpv Typing Of Low Prevalent Types

Autori
Sabol, Ivan ; Salakova, Martina ; Smahelova, Jana ; Milutin Gasperov, Nina ; Tachezy, Ruth ; Grce, Magdalena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
23rd International Papillomavirus Conference & Clinical Workshop : Book of abstracts / Hamsikova, Eva ; Haugen, Thomas H ; Palefsky, Joel ; Smith, Elaine M ; Tachezy, Ruth ; Turek, Lubomir P ; Vonka, Vladimir - Prag : The Institute of Hematology and Blood Transfusion, 2006, 370-370

Skup
International Papillomavirus Conference & Clinical Workshop (23 ; 2006)

Mjesto i datum
Prag, Češka Republika, 01.-07.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
HPV

Sažetak
There are more than 100 human papillomavirus (HPV) types and approximately 40 infect the anogenital tract. In cervical precancerous lesions (LSIL and HSIL, low and high grade squamous intraepithelial lesions), a large variety of HPV types can be found, while in cervical cancer only high-risk (HR) or HR together with low-risk (LR) types. Many different techniques are currently being used for HPV detection and typing in clinical samples. The vaccination against HPV is becoming a reality in the nearest future and typing of HPV will be an important tool in the monitoring of vaccine efficiency. Therefore, the performance of different methods of HPV typing needs to be assessed. For this study, cervical samples of Croatian and Czech women diagnosed with HSIL and previously collected and HPV tested were used. Only HPV positive samples for types other than the most common HPV types 6/11, 16, 18, 31 and 33 or samples positive for multiple HPV types were selected. The detection and typing of HPV was done by consensus MY09/11 and type-specific (TS) PCR (Croatia) and by GP5+/6+ PCR and reverse-line blot assay (RLB) (Czech Republic). All samples were further analyzed by Line Probe Assay (LiPA) (Innogenetics) and Linear Array (LA) (Roche). The results of the performance of different techniques and comparison of interlaboratory results were statistically analyzed. All samples were successfully typed by LA (100%), while LiPA and RLB method did not reveal the type in all samples. In more than 50% samples, all 3 tests agree on at least one HPV type. In approximately 19% cases the results of all tests were in agreement even when multiple types were present. The discordant results were detected in 6% of samples. Larger series of the samples will be evaluated for poster presentation. Our preliminary data shows that LA system can more frequently detect low prevalent HPV types than LiPA and RLB.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
0098104

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Magdalena Grce (autor)

Avatar Url Nina Milutin Gašperov (autor)

Avatar Url Ivan Sabol (autor)


Citiraj ovu publikaciju:

Sabol, Ivan; Salakova, Martina; Smahelova, Jana; Milutin Gasperov, Nina; Tachezy, Ruth; Grce, Magdalena
Evaluation Of Different Techniques For Hpv Typing Of Low Prevalent Types // 23rd International Papillomavirus Conference & Clinical Workshop : Book of abstracts / Hamsikova, Eva ; Haugen, Thomas H ; Palefsky, Joel ; Smith, Elaine M ; Tachezy, Ruth ; Turek, Lubomir P ; Vonka, Vladimir (ur.).
Prag: The Institute of Hematology and Blood Transfusion, 2006. str. 370-370 (poster, nije recenziran, sažetak, znanstveni)
Sabol, I., Salakova, M., Smahelova, J., Milutin Gasperov, N., Tachezy, R. & Grce, M. (2006) Evaluation Of Different Techniques For Hpv Typing Of Low Prevalent Types. U: Hamsikova, E., Haugen, T., Palefsky, J., Smith, E., Tachezy, R., Turek, L. & Vonka, V. (ur.)23rd International Papillomavirus Conference & Clinical Workshop : Book of abstracts.
@article{article, author = {Sabol, Ivan and Salakova, Martina and Smahelova, Jana and Milutin Gasperov, Nina and Tachezy, Ruth and Grce, Magdalena}, year = {2006}, pages = {370-370}, keywords = {HPV}, title = {Evaluation Of Different Techniques For Hpv Typing Of Low Prevalent Types}, keyword = {HPV}, publisher = {The Institute of Hematology and Blood Transfusion}, publisherplace = {Prag, \v{C}e\v{s}ka Republika} }
@article{article, author = {Sabol, Ivan and Salakova, Martina and Smahelova, Jana and Milutin Gasperov, Nina and Tachezy, Ruth and Grce, Magdalena}, year = {2006}, pages = {370-370}, keywords = {HPV}, title = {Evaluation Of Different Techniques For Hpv Typing Of Low Prevalent Types}, keyword = {HPV}, publisher = {The Institute of Hematology and Blood Transfusion}, publisherplace = {Prag, \v{C}e\v{s}ka Republika} }




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