engleski

CELLULAR AND INTRACELLULAR RESPONSE TO ELECTROMAGNETIC FIELD OF ULTRA HIGH FREQUENCY RADIATION

The aim of this study was to evaluate whether low-level, ultra high frequency (UHF) irradiation of 935 MHz influences the cytoskeleton and the cell growth of V79 cells. UHF field was generated inside a Gigahertz Transversal Electromagnetic Mode cell (GTEM-cell) equipped with a Hewlett Packard signal generator. The electric field strength was 8.2&#177; 0.3 V/cm, and the average specific absorption rate (SAR) was calculated to be 0.12 W/kg. Cell samples were cultivated in a humid atmosphere at 37 °C in 5% CO2. Prepared cell samples were exposed to the 935 MHz continuous wave frequency field for 1, 2 and 3 hours. The structure of microtubule proteins was determined using the immunocytochemical method. Cell growth was determined by counting cells for each hour of exposure during five post-exposure days. The experimental procedure included exposed V79 and unexposed control cells. In comparison to the control cells, the microtubule structure of the exposed cells significantly altered after 3 hours of irradiation (p<0.05). Cell growth of the cells exposed for three hours significant dropped 72 hours after exposure (p<0.05). It could be concluded that the 935 MHz low-level UHF radiation affected microtubule proteins, consequently influencing the cell growth.

Cell growth; microtubule; 935 MHz; GTEM-cell

nije evidentirano