Overexpression of Phenylalanine ammonia-lyase and Rosmarinic Acid Production in Transgenic Roots of Coleus blumei Benth. (CROSBI ID 520369)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Bauer, Natasa ; Fulgosi, Hrvoje ; Leljak-Levanić, Dunja ; Jelaska, Sibila
engleski
Overexpression of Phenylalanine ammonia-lyase and Rosmarinic Acid Production in Transgenic Roots of Coleus blumei Benth.
Rosmarinic acid (RA) is common caffeic acid ester in the plant kingdom. In plants it acts as a preformed constitutively accumulated defense compound. It is biosynthesized via the phenyl-propanoid pathway. The eight enzymes involved in the biosynthesis are known and characterized. Precursor for the caffeic acid moiety of RA is L-phenylalanine. Phenylalanine ammonia-lyase (PAL) catalyzes the oxidative desamination of L-phenylalanine. Previous results indicate that induction of PAL activity is the consequence of transcriptional activation of PAL genes. The correlation of the PAL activity with RA synthesis is noticed. To get more knowledge on that correlation, activity of phenylalanine ammonia-lyase, in transgenic roots of Coleus blumei Benth. was perturbed by introduction of an Arabidopsis thaliana PAL1 gene controlled by the CaMV 35S promoter. Thirteen transgenic root lines resistant to gluphosinate ammonium (selective marker) were established and maintained on MS medium supplemented with 5.4  M NAA. The content of RA in root cultures was quantified by HPLC, and PAL activity was measured spectrophotometricaly on 300 nm with L-phenylalanine as a substrate. Comparing with control-normal root line, all 13 transgenic lines had strong enhancement of PAL activity that was reflected on induction of phenolic production. Rosmarinic acid accumulation was significantly induce in 8 lines. In one transgenic root line RA production was diminished and greater amount of chlorogenic acid was noticed.
Coleus blumei; rosmarinic acid; transformation; Agrobacterium; phenylalanine ammonia-lyase
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Podaci o prilogu
157-x.
2006.
objavljeno
Podaci o matičnoj publikaciji
11th IAPTC&B Congress, Biotechnology and Sustainable Agriculture 2006 and Beyond, Abstracts
Pua, Eng-Chong
Peking:
Podaci o skupu
11th IAPTC&B Congress "Biotechnology and Sustainable Agriculture 2006 and Beyond"
poster
13.08.2006-18.08.2006
Peking, Kina