engleski

MATURE CD83+ DENDRITIC CELLS ACTIVATE DECIDUAL NK CELLS

Problem: The influence of decidual dendritic cells (DC) on decidual NK cells was investigated. Material and methods: Distribution of CD83+ cells in early pregnancy decidua was analysed in comparison with trophoblast invasion and NK cell-infiltration using immunohistology. Phenotype, origin, maturation state and cytokine expression of DC were detected by flow cytometry. Magnetically purified CD56+ and CD83+ cells were co-cultured. Proliferation CFSE assay, CD69 and cytolytic mediators expression in NK cells were analysed. Results: CD83+DC were found in the vicinity of NK cells at the area of trophoblast invasion and expressed CD86, HLA-DR and CD11c in vitro. DC expressed also IL-15, IL-18 and IFNgamma. CD83+DC increased proliferation, CD69 and cytolytic mediator expression in decidual NK cells. Conclusion: Mature decidual DC of mostly myeloid origin are capable to activate decidual NK cells in vitro. The investigation was supported by Croatian Ministry of Science, Grant No. 0062029 and European FVI Project (EMBIC, LSHM-CT-2004-512040, NoE).

Dendritic cells; NK cells; Pregnancy

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