engleski

Different distribution of glycosphingolipids in mouse and rabbit skeletal-muscle demonstrated by biochemical and immunohistological analyses

Glycosphingolipids (GSLs) are ubiquitious components of the outer surface of animal cell membranes. Their localization suggests that they are involved in several membrane-mediated phenomena. In this study we describe the structural characterization of GSLs from mouse and rabbit skeletal muscle by biochemical analyses and their localization in muscle tissue using immunohistochemical methods. Neutral GSLs of rabbit muscle showed a simple TLC pattern expressing mainly monohexosylceramide (MHC). In addition to MHC, lactosylceramide, lacto-N-neotetraosyl ceramide, globoside and Forssman-GSL were detected in the neutral GSL fraction of mouse muscle by TLC-immunostaining (overlay technique) with specific monoclonal and polyclonal antibodies. The major ganglioside in both species was GM3 (Neu5Ac) whereas GM3(Neu5Ac)and GM3(Neu5Gc) were found in a 1.85:1 ratio in mouse muscle. Sialyl lacto-N-neotetraosyl ceramide and sialyl lacto-N-norhexaosyl ceramide were found in both animals in relatively high concentrations. Furthermore, minor amounts of the ganglio-series gangliosides GM1, GD1a, GD1b and GT1b were detected in muscles of both species. GSL expression, determined by chromatographic analysis and TLC-immunostaining could be confirmed immunohistochemically by examining frozen sections of skeletal muscle samples from both species. The results provide the basis for the investigation of specific GSLs that migh modulate membrane protein function in muscle.

glycosphingolipids; mouse and rabbit skeletal muscle; immunohistochemisry

nije evidentirano