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Pregled bibliografske jedinice broj: 260759

Human gliosarcoma-associated ganglioside composition is complex and distinctive as evidenced by high-performance mass spectrometric determination and structural characterization


Vukelić, Željka; Kalanj-Bognar, Svjetlana; Froesch, Martin; Bindila, Laura; Radić, Boris; Allen, Mark; Peter-Katalinić, Jasna; Zamfir, Alina D.
Human gliosarcoma-associated ganglioside composition is complex and distinctive as evidenced by high-performance mass spectrometric determination and structural characterization // Glycobiology, 17 (2007), 5; 504-515 doi::10.1093/glycob/cwm012 (međunarodna recenzija, članak, znanstveni)


Naslov
Human gliosarcoma-associated ganglioside composition is complex and distinctive as evidenced by high-performance mass spectrometric determination and structural characterization

Autori
Vukelić, Željka ; Kalanj-Bognar, Svjetlana ; Froesch, Martin ; Bindila, Laura ; Radić, Boris ; Allen, Mark ; Peter-Katalinić, Jasna ; Zamfir, Alina D.

Izvornik
Glycobiology (0959-6658) 17 (2007), 5; 504-515

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Biomarkers and target molecules; Ganglioside structures; Gliosarcoma ganglioside composition; High-performance mass spectrometry; Novel O-acetylated GD3 isomer
(Gliosarcoma ganglioside composition; High-performance mass spectrometry; Novel O-acetylated GD3 isomer)

Sažetak
Gangliosides (GGs), involved in malignant alteration and tumor progression/invasiveness, are considered as tumor biomarkers or therapeutic targets. Here, we describe the first systematic GG composition characterization in human gliosarcoma versus normal brain tissue using our recently developed mass spectrometry (MS) methods, based on nano-electrospray (nano-ESI), Fourier-transform ion cyclotron resonance (FT-ICR), and chip nano-ESI quadrupole time-of-flight (QTOF), complemented by thin-layer chromatographic (TLC) analysis and quantification. Combined MS enabled detection and structural assignment of 73 distinct GG species: many more than reported so far for investigated gliomas. Apart from the 7.4-times lower total GG content, gliosarcoma contained all major brain-associated species, however, in very altered proportions, exhibiting a highly distinctive pattern: GD3 (48.9%) > GD1a/nLD1 > GD2/GT3 > GM3 > GT1b > GM2 > GM1a/GM1b/nLM1 > LM1 > GD1b > GQ1b. MS also revealed abundant O-Ac-GD3 ; its sequencing provided structural evidence to postulate a novel O-Ac-GD3 isomer O-acetylated at the inner Neu5Ac-residue, previously not structurally confirmed. The high sensitivity and mass accuracy permitted the assignment of unusual minor species: GM4, Hex-HexNAc-nLM1, Gal-GD1, Fuc-GT1, GalNAc-GT1, O-Ac-GM3, di- O-Ac-GD3O-Ac-GD3, and O-Ac-GT3, not previously reported as glioma-associated. The gliosarcoma-expressed GA2 might represent a marker distinguishing astrocytic from oligodendroglial tumors. This is, to our knowledge, so far the most complete GG composition characterization of certain glioma, which demonstrates that our MS-based approach could provide essential structural information relevant to glycosphingolipid role(s) in brain tumor biology, differential diagnosis/prognosis and novel treatment concepts.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1081870-1877 - Uloga membranskih lipida u moždanom razvitku, starenju i neurodegeneraciji (Svjetlana Kalanj-Bognar, )
108-1081870-2415 - Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije (Željka Vukelić, )

Ustanove
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • BIOSIS Previews (Biological Abstracts)
  • EMBASE (Excerpta Medica)
  • MEDLINE
  • Science Citation Index


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