Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 25989

Big endotehelin-1 stimulates 1, 2 - diacylglycerol generation in the rat proximal tubule independently from the conversion into endothelin-1


Knotek, Mladen; Beara-Lasić, Lada; Čejvan, Kenan; Jakšić, Ozren; Lasić, Zoran; Skorić, Boško; Banfić, Hrvoje
Big endotehelin-1 stimulates 1, 2 - diacylglycerol generation in the rat proximal tubule independently from the conversion into endothelin-1 // The 2nd European Kidney Research Forum : abstracts / Thurau, Klaus ; Remuzzi, Giuseppe (ur.).
Baveno, 1996. (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 25989 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Big endotehelin-1 stimulates 1, 2 - diacylglycerol generation in the rat proximal tubule independently from the conversion into endothelin-1

Autori
Knotek, Mladen ; Beara-Lasić, Lada ; Čejvan, Kenan ; Jakšić, Ozren ; Lasić, Zoran ; Skorić, Boško ; Banfić, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The 2nd European Kidney Research Forum : abstracts / Thurau, Klaus ; Remuzzi, Giuseppe - Baveno, 1996

Skup
European Kidney Research Forum (2 ; 1996)

Mjesto i datum
Baveno, Italija, 24.-25.05.1996

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
big endothelin-1; endothelin-1; 1; 2-diacylglycerol; rat proximal tubule

Sažetak
Endothelin-1 (ET-1) is formed from its precursor big ET-1by the action of ednothelin-converting enzyme (ECE). Unlike the renal vascular effects of big ET-1, tubular responses to big ET-1 in the rat kidney were demonstrated to be only partially sensitive to an ECE inhibitor phosphoramidon (Pho). In our previous study we demonstrated in the rat proximal tubule (PT) ET-dependent stimulation of phospholipase C trough the ET(B) receptors in the brush-border membrane (BBM), and ET(C) receptors in the basolateral membrane. In the present study the relationship between the functional response to big ET-1 and its conversion to the ET-1 in rat PT was assessed. 10 min incubation of renal cortical slices with 10(-7) M big ET-1 resulted in 712+96 fmol ET-1/mg tissue. In the slice pretreated with Pho 10(-4) M, tissue ET-1 content following 10 min incubation with 10(-7) M big ET-1 was reduced (309+12 fmol ET-1/mg tissue, p<0.05 vs. big ET-1 + Pho), demonstration the presence and the efficacy of inhibition of the ECE. Following incubation of renal cortical slices with big ET-1, dose- and time-dependent increment in 1, 2 -diacylglycerol (DAG) was observed in BBM. There was no increment in DAG in response to big ET-1 in BLM. Following 7 min incubation of renal coritcal slices with 10(-7) M big endothelin-1, DAG concentration in BBM increased from 317+6 pmol/mg prot. (p<0.001). This increment in DAG in the BBM was insensitive to the pretreatment with 10(-4) M Pho (438+6 pmol/mg prot., n.s. compared to big ET-1 alone). We conclude that in the rat PT, big ET-1 dependent signalling may occur independently from its prior enyzmatic conversion into ET-1. BBM may probably be the site of big ET-1-sensitive receptors.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekti:
108013

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Mladen Knotek (autor)

Avatar Url Ozren Jakšić (autor)

Avatar Url Hrvoje Banfić (autor)

Avatar Url Boško Skorić (autor)


Citiraj ovu publikaciju:

Knotek, Mladen; Beara-Lasić, Lada; Čejvan, Kenan; Jakšić, Ozren; Lasić, Zoran; Skorić, Boško; Banfić, Hrvoje
Big endotehelin-1 stimulates 1, 2 - diacylglycerol generation in the rat proximal tubule independently from the conversion into endothelin-1 // The 2nd European Kidney Research Forum : abstracts / Thurau, Klaus ; Remuzzi, Giuseppe (ur.).
Baveno, 1996. (poster, međunarodna recenzija, sažetak, znanstveni)
Knotek, M., Beara-Lasić, L., Čejvan, K., Jakšić, O., Lasić, Z., Skorić, B. & Banfić, H. (1996) Big endotehelin-1 stimulates 1, 2 - diacylglycerol generation in the rat proximal tubule independently from the conversion into endothelin-1. U: Thurau, K. & Remuzzi, G. (ur.)The 2nd European Kidney Research Forum : abstracts.
@article{article, author = {Knotek, Mladen and Beara-Lasi\'{c}, Lada and \v{C}ejvan, Kenan and Jak\v{s}i\'{c}, Ozren and Lasi\'{c}, Zoran and Skori\'{c}, Bo\v{s}ko and Banfi\'{c}, Hrvoje}, year = {1996}, pages = {P064}, keywords = {big endothelin-1, endothelin-1, 1, 2-diacylglycerol, rat proximal tubule}, title = {Big endotehelin-1 stimulates 1, 2 - diacylglycerol generation in the rat proximal tubule independently from the conversion into endothelin-1}, keyword = {big endothelin-1, endothelin-1, 1, 2-diacylglycerol, rat proximal tubule}, publisherplace = {Baveno, Italija} }
@article{article, author = {Knotek, Mladen and Beara-Lasi\'{c}, Lada and \v{C}ejvan, Kenan and Jak\v{s}i\'{c}, Ozren and Lasi\'{c}, Zoran and Skori\'{c}, Bo\v{s}ko and Banfi\'{c}, Hrvoje}, year = {1996}, pages = {P064}, keywords = {big endothelin-1, endothelin-1, 1, 2-diacylglycerol, rat proximal tubule}, title = {Big endotehelin-1 stimulates 1, 2 - diacylglycerol generation in the rat proximal tubule independently from the conversion into endothelin-1}, keyword = {big endothelin-1, endothelin-1, 1, 2-diacylglycerol, rat proximal tubule}, publisherplace = {Baveno, Italija} }




Contrast
Increase Font
Decrease Font
Dyslexic Font