engleski

Correlation of structure and retention behaviour in reversed-phase high-performance liquid chromatography. I. Leucine-enkephalin-related glycoconjugates

The chromatographic behaviour of leucine-enkephalin-related glycoconjugates with an ester-, ether- and amide-type of linkage was investigated by reversed-phase high-performance liquid chromatography using trifluoroacetic acid as ion-pairing agent and methanol as modifier of the aqueous phase. The results show that the position and the type of sugar-peptide linkage, the type of sugar moiety introduced and the degree of carbohydrate protection contribute to the overall retention of the glycopeptides studied.

HPLC ; retention ; glycopeptide ; enkephalin

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