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Pregled bibliografske jedinice broj: 258256

Enzyme-linked immunosorbent assay for 4-hydroxynonenal– histidine conjugates


Borović, Suzana; Rabuzin, Filip; Waeg, Georg; Žarković, Neven
Enzyme-linked immunosorbent assay for 4-hydroxynonenal– histidine conjugates // Free Radical Research, 40 (2006), 809-820 (međunarodna recenzija, članak, znanstveni)


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Naslov
Enzyme-linked immunosorbent assay for 4-hydroxynonenal– histidine conjugates

Autori
Borović, Suzana ; Rabuzin, Filip ; Waeg, Georg ; Žarković, Neven

Izvornik
Free Radical Research (1071-5762) 40 (2006); 809-820

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
oxidative stress; protein modification; 4-hydroxynonenal; indirect ELISA

Sažetak
Highly reactive aldehyde 4-hydroxynonenal (HNE) is the final product of lipid peroxidation, known as a second messenger of free radicals and a signaling molecule. It forms protein conjugates involved in pathology of various diseases. To determine cellular HNE– protein conjugates we developed indirect ELISA based on well-known, monoclonal antibody against HNE– histidine (HNE– His) adducts. The method was calibrated using HNE– albumin conjugates as standards (R2 = 0.999) and validated on human osteosarcoma cell cultures (HOS). The ELISA showed good sensitivity (8.1 pmol HNE– His/mg of protein), precision ( &plusmn ; 8% intra-assay and &plusmn ; 12% inter-assay) and spiking recovery ( &plusmn ; 9%). The assay revealed 60-fold increase of cellular HNE– His adducts upon copper-induced lipid peroxidation of HOS. The ELISA matched HNE-immunocytochemistry of HNE-treated HOS cells and quantified the increase of cellular HNE– His conjugates in parallel to the decrease of free HNE in culture medium. The ELISA was developed as ELISA Stress for severe lipid peroxidation and ELISA Fine for studies on HNE physiology.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
0098101

Ustanove:
Institut "Ruđer Bošković", Zagreb


Citiraj ovu publikaciju

Borović, Suzana; Rabuzin, Filip; Waeg, Georg; Žarković, Neven
Enzyme-linked immunosorbent assay for 4-hydroxynonenal– histidine conjugates // Free Radical Research, 40 (2006), 809-820 (međunarodna recenzija, članak, znanstveni)
Borović, S., Rabuzin, F., Waeg, G. & Žarković, N. (2006) Enzyme-linked immunosorbent assay for 4-hydroxynonenal– histidine conjugates. Free Radical Research, 40, 809-820.
@article{article, year = {2006}, pages = {809-820}, keywords = {oxidative stress, protein modification, 4-hydroxynonenal, indirect ELISA}, journal = {Free Radical Research}, volume = {40}, issn = {1071-5762}, title = {Enzyme-linked immunosorbent assay for 4-hydroxynonenal and \#8211; histidine conjugates}, keyword = {oxidative stress, protein modification, 4-hydroxynonenal, indirect ELISA} }
@article{article, year = {2006}, pages = {809-820}, keywords = {oxidative stress, protein modification, 4-hydroxynonenal, indirect ELISA}, journal = {Free Radical Research}, volume = {40}, issn = {1071-5762}, title = {Enzyme-linked immunosorbent assay for 4-hydroxynonenal and \#8211; histidine conjugates}, keyword = {oxidative stress, protein modification, 4-hydroxynonenal, indirect ELISA} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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