engleski

Application of Optical Reflectometry for Characterization of Polyelectrolyte-Protein Multilayers

It is known that the build-up of polyelectrolyte multilayers can be followed in situ by means of stagnation point optical reflectometry. The application of optical reflectometry for characterization of multilayers obtained by exposing a suitable substrate to solutions of a cationic homopolymer and an oppositely charged protein was investigated. In all experiments, as negatively charged component Bovine Serum Albumine was used. As the cationic homopolymer, a weak polyelectrolyte poly(dimethylaminoethyl)methacrylate (PAMA) or a strong polyelectrolyte poly(2-vinyl-n-methylpyridinium iodide) (PVP+) were used. As the solid substrate, we used silica in the form of silicon wafers carrying an oxide layer and as the supporting electrolyte we used potassium chloride or phosphate buffer. The influence of ionic strength, pH, type of cationic homopolymer (strong or weak), and protein concentration were investigated. It was shown that stagnation point optical reflectometry can be a useful method for characterization of formation of multilayers containing polyelectrolyte and protein layers.

polyelectrolytes; proteins; optical reflectometry; multilayers

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