engleski

A comparison of peritoneal exudate cells and peripheral blood leucocytes in direct and indirect migration inhibition tests as an in vitro assay for tuberculin hypersensitivity in guinea pigs

Peritoneal exudate cells (PEC) and peripheral blood leukocytes (PBL) are most frequently used in the migration inhibition test. The aim of this work was to compare the ability of these two types of cells to reflect ruberculin hypersensitivity in the migration inhibition test. We sensitized 36 guinea pigs with complete Freund's adjuvant and 20 controls were injected with incomplete Freund's adjuvant. Migration of PEC in medium containing 5, 15, or 75  g of PPD/ml was assessed after 30 min, and 1, 2, 4 18, 24, and 48 hr of incubation. The migration of PEC from sensitized animals was inhibited, the inhibition being dose dependent and, with lower concentrations of the antigen, becoming significant only after 4 hr or later. With both PEC and PBL from the same sensitized animal we observed virtually identical migration inhibition in the presence of 75  g of PPD/ml. A correlation was found between the migration inhibition indices of PEC and PBL. In the indirect test, active supernatants containing lymphokines caused nearly identical migration inhibition of PEC and PBL from normal animals. It follows that in the guinea pig PEC and PBL behave alike both in the direct and indirect migration inhibition test. Thus, PEC and PBL appear to be equally valuable sources of cells for migration inhibition tests.

delayed hypersensitivity ; tuberculin ; in vitro ; migration inhibition ; peritoneal exudate ; peripheral blood ; skin test ; guinea pig

Rad je prezentiran na skupu i kao sažetak objavljen u Periodicum biologorum 78 (1976) (Suppl.) 61.