engleski

Wheat auxin-amidohydrolase: a regulator of auxin activity in plants

The wheat auxin-amidohydrolase, TaIAR3, was investigated. TaIAR3 cDNA, isolated from winter wheat (Triticum aestivum L. cv. Caledonia), was obrained by courtesy of Dr. James Campanella, Montclair State University, NJ, USA, and Dr. Jutta Ludwig-Mueller, Institut fuer Botanik, Dresden, Germany. The wheat auxin-amidohydrolase gene was cloned into the pGEX4T-1 expression vector and protein expression was obtained in E. coli. The GST-TaIAR3 protein was purified by chromatography on glutathione-Sepharose 4B and was processed for biochemical reaction with auxin-amino acid conjugates as putative substrates. The auxin formed was monitored by HPLC. HPLC separation showed cleavage of the, biologically inactive, alanine conjugate of indolebutyric acid (IBA-Ala) to form the, physiologically active, free IBA. This fact suggests that wheat auxin-amidohydrolase is involved in auxin homeostasis in plants.

Triticum aestivum L. cv. Caledonia; winter wheat; auxin amidohydrolase; TaIAR3; protein expression in Escherichia coli; enzymatic activity; indolebutyric acid; IBA; IBA-alanine

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