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Pregled bibliografske jedinice broj: 256612

Photophysics of the Fluorescent pH Indicator BCECF


Boens, Noël; Qin, Wenwu; Basarić, Nikola; Orte, Angel; Talavera, Eva M.; Alvarez-Pez, Jose M.
Photophysics of the Fluorescent pH Indicator BCECF // The Journal of Physical Chemistry A, 110 (2006), 30; 9334-9343 (međunarodna recenzija, članak, znanstveni)


Naslov
Photophysics of the Fluorescent pH Indicator BCECF

Autori
Boens, Noël ; Qin, Wenwu ; Basarić, Nikola ; Orte, Angel ; Talavera, Eva M. ; Alvarez-Pez, Jose M.

Izvornik
The Journal of Physical Chemistry A (1089-5639) 110 (2006), 30; 9334-9343

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Absorption; BCECF; fluorescence; global analysis; photophysics; pH

Sažetak
The photophysical behavior of BCECF [2', 7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein] − currently the most widely used fluorescent pH indicator for near-neutral intracellular pH measurements – has been explored by using absorption and steady-state and time-resolved fluorescence measurements. The influence of ionic strength as well as total buffer concentration on the absorbance and steady-state fluorescence has been investigated. The apparent acidity constant of the pH indicator determined by absorbance and fluorescence titration is dependent on the added buffer and salt concentrations. A semi-empirical model is proposed to rationalize the variations in the apparent pKa values. The excited-state proton exchange of BCECF at physiological pH becomes reversible upon addition of phosphate buffer inducing a pH dependent change of the fluorescence decay times. Fluorescence decay traces collected as a function of total buffer concentration and pH were analyzed by global compartmental analysis yielding the following values of the rate constants describing excited-state dynamics of BCECF: k01 = 3.4 x 108 s− 1, k02 = 2.6 x 108 s− 1, k21 = 1 x 106 M− 1 s− 1, = 1.4 x 108 M− 1 s− 1, and = 4.3 x 107 M− 1 s− 1.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
0098052

Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Nikola Basarić, (250000)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Chemical Abstracts