engleski

Di-, tri-, and tetrameric building blocks with carbamate internucleoside linkages for oligonucleotides synthesis

Synthesis of di-, tri-, and tetrameric building blocks with carbamate internucleoside linkages was achieved by reaction of mono-, di- or trimeric nucleoside 5^'-O-p-nitrophenyl carbonate intermediates with 3^'-amino-3^'-deoxythymidine. These building blocks were suitably protected at 5^'-position and converted into phosphoramidites, or attached onto CPG, and then used for the chimeric oligonucleotides synthesis. These chimeric oligonucleotides have been studied for their binding properties to complementary nucleic acids and for their nuclease sensitivity.

carbamate internucleoside linkages

nije evidentirano