Enzyme-linked immunosorbent assay for 4-hydroxynonenal-protein conjugates (CROSBI ID 518450)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Borović, Suzana ; Rabuzin, Filip ; Waeg, Georg ; Žarković, Neven
engleski
Enzyme-linked immunosorbent assay for 4-hydroxynonenal-protein conjugates
Highly reactive aldehyde 4-hydroxynonenal (HNE) is the final product of lipid peroxidation, known as a second messenger of free radicals and a signaling molecule. It forms protein conjugates involved in pathology of various diseases. To determine cellular HNE– protein conjugates we developed indirect ELISA based on well-known, genuine monoclonal antibody against HNE– histidine (HNE– His) adducts. The method was calibrated using HNE– albumin conjugates as standards (R2 = 0.99) and validated on human osteosarcoma cell cultures (HOS). The ELISA showed good sensitivity (8.1 pmol HNE– His/mg of protein), precision (8% intra-assay and 12% inter-assay) and spiking recovery (9%). The assay revealed 60-fold increase of cellular HNE– His adducts upon copper-induced lipid peroxidation of HOS. The ELISA matched HNE-immunocytochemistry of HNE-treated HOS cells and quantified the increase of cellular HNE– His conjugates in parallel to the decrease of free HNE in culture medium. The ELISA was developed as ELISA Stress for severe lipid peroxidation and ELISA Fine for studies on HNE physiology.
4-hydroxynonenal; ELISA
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Podaci o prilogu
20-20.
2006.
objavljeno
Podaci o matičnoj publikaciji
HNE-Club Meeting : Book of abstracts
Podaci o skupu
HNE-Club Meeting
predavanje
16.06.2006-18.06.2006
Genova, Italija