engleski

Isolation and study of the composition of a peptidoglycan complex excreted by the biotin-requiring mutant of Brevibacterium divaricatum NRRL-2311 in the presence of penicillin

When cultures of biotin-requiring mutant of Brevibacterium divaricatum NRRL-2311 were treated in early logarithmic phase with penicillin in a glucose-mineral medium under excess supply of biotin, large amounts of high molecular weight material accumulated in the medium. The analyses of the excreted material showed that the main constituents were peptidoglycan components of noncross linked structure bearing both L- and D-alanine residues ; evidence was also obtained for the occurence of extractable lipid material, non-amino sugars and organic phosphate. Several procedures were examined for the purification of the peptidoglycan complex. Suitable labelling experiments with 14-C-Glu and analyses of lysozyme digests of the purified fractions , suggest that somes of the components might contain murein fragments covalently linked to a polysaccharide portion.

Peptidoglycan ; Brevibacterium divaricatum ; inhibition with penicillin ; 14-C-labelled peptidoglycan ; lysozyme digests

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