engleski

Isolation procedure and properties of monomer unit from lysozyme digest of peptidoglycan complex excreted into the medium by penicillin-treated Brevibacterium divaricatum mutant

The large peptidoglycan complex was excreted into the medium by the biotin-requiring mutant of Brevibacterium divaricatum NRRL-2311 incubated in the presence of penicillin for 1 hr. The released peptidoglycan possesses the linear uncrosslinked structure made of repeating disaccharide-pentapeptide units. The complex was isolated from culture fluid by stepwise gel filtration. A convenient isolation procedure with high yield for the pure monomeric unit from lysozyme digest of the accumulated polymer is described. 14-C-labeled monomer units were prepared.

Peptidoglycan ; peptidoglycan monomer ; penicillin treatment ; lysozyme digest

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