engleski

The metabolic fate of 14C-labeled immunoadjuvant peptidoglycan monomer. II. In Vitro studies

Peptidoglycan monomer, GlcNAc-MurNAc-L-Ala-D-isoGln-mesoDAP-D-Ala-D-Ala (PGM), labeled with 14-C both in the disaccharide and pentapeptide portions, was incubated with slices of mouse liver, kidney or spleen as well as with mouse and human blood, blood cells, plasma and serum. PGM was isolated unchanged after incubations with mouse organs and blood cells. However, upon incubation with mouse or human blood, 10-50% of the PGM underwent hydrolysis to the corresponding disaccharide and pentapeptide. After incubations with plasma and serum more than 90% of the 14-C-PGM was metabolized: about 50% of the administered radioactive dose was recovered in the disaccharide unit and about 35% in the peptide part. These results suggest that in blood, plasma and serum of mouse and man, an N-acetylmuramyl-L-alanine amidase exists which splits the amide bond between the lactyl carboxyl group of the muramyl residue and the aminogroup of the peptide moiety in the peptidoglycan molecule

Peptidoglycan monomer ; muramyl dipeptide ; N-acetylmuramyl-L-alanine amidase

nije evidentirano