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Partial purification and characterization of N-Acetylmuramyl-L-alanine amidase from human and mouse serum.


Valinger, Zdenka; Ladešić, Branko; Tomašić, Jelka
Partial purification and characterization of N-Acetylmuramyl-L-alanine amidase from human and mouse serum. // Biochimica at Biophysica Acta, 701 (1982), 63-71 (međunarodna recenzija, članak, znanstveni)


Naslov
Partial purification and characterization of N-Acetylmuramyl-L-alanine amidase from human and mouse serum.

Autori
Valinger, Zdenka ; Ladešić, Branko ; Tomašić, Jelka

Izvornik
Biochimica at Biophysica Acta (0167-4838) 701 (1982); 63-71

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Peptidoglycan monomer; N-Acetylmuramyl-L-alanine amidase. mammalian sera
(Peptidoglycan monomer; N-Acetylmuramyl-L-alanine amidase; mammalian sera)

Sažetak
The enzyme N-acetylmuramyl-L-alanine amidase has been detcted in human, mouse, rabbit, bovine and sheep sera. A method for detection of amidase activity using 14-C-peptidoglycan monomer as the substrate has been developed. Partial purification of human and mouse amidase was achieved by gel chromatography. Both amidase preparations exhibited maximal activity at pH 9.0 in Tris-HCl buffer and required Mg2+ for maximal activity. Following digestion of peptidoglycan monomer, the disaccharide GlcNAc-MurNAc and the corresponding pentapeptide L-Ala-D-isoGln-meso-DAP-D-Ala-D-Ala were formed and subsequently isolated and chemically characterized.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Valinger, Zdenka; Ladešić, Branko; Tomašić, Jelka
Partial purification and characterization of N-Acetylmuramyl-L-alanine amidase from human and mouse serum. // Biochimica at Biophysica Acta, 701 (1982), 63-71 (međunarodna recenzija, članak, znanstveni)
Valinger, Z., Ladešić, B. & Tomašić, J. (1982) Partial purification and characterization of N-Acetylmuramyl-L-alanine amidase from human and mouse serum.. Biochimica at Biophysica Acta, 701, 63-71.
@article{article, year = {1982}, pages = {63-71}, keywords = {Peptidoglycan monomer, N-Acetylmuramyl-L-alanine amidase, mammalian sera}, journal = {Biochimica at Biophysica Acta}, volume = {701}, issn = {0167-4838}, title = {Partial purification and characterization of N-Acetylmuramyl-L-alanine amidase from human and mouse serum.}, keyword = {Peptidoglycan monomer, N-Acetylmuramyl-L-alanine amidase, mammalian sera} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus