Increased genotoxicity of acetylaminofluorene by modulators of multixenobiotic resistance mechanism: studies with the fresh water clam Corbicula fluminea (CROSBI ID 124378)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Waldman, Petra ; Pivčević, Branka ; Müller, Werner E. G. ; Zahn, Rudolf K. ; Kurelec, Branko
engleski
Increased genotoxicity of acetylaminofluorene by modulators of multixenobiotic resistance mechanism: studies with the fresh water clam Corbicula fluminea
The presence of a ‘multixenobiotic resistance’ [MXR] mechanism in gills of the freshwater clam Corbicula flumenea was investigated. Western blot analyses of membrane vesicles from gills, applying antibodies to vertebrate P170 multidrug resistance (MDR) protein, revealed a 135 kDa immunoreactive protein. Verapamil caused a reduction of 3H-vincristine (3H-VCR) binding onto vesicles from calm. Exposure of calms to 3H-VCR in the presence of verapamil or staurosporine (STP) enhanced the accumulation of 3H-VCR over control values. Furthermore, clams were exposed instead to VCR, to a model carcinogen, 2-acetylaminofluorene (AAF), to determine the verapamil- and STP-dependent increase of single-strand breaks (SSBs) in DNA from gills of this organism. Verapamil caused no or little increase of SSBs induced by exposure to 0.01 or 0.10 μ M AAF, respectively, as measured by the alkaline elution technique. In contrast, in the presence of STP a highly significant and dose-dependent enhancement of AAF-mediated SSBs was measured already at exposure to 0.01 μ M AAF. These data indicate (i) that the calm C. fluminea is provided with a P-glycoprotein-like element of the MDR-mechanism, (ii) that this system can be poisoned by chemosensitizers such as verapamil and STP, (iii) the role of protein kinase C in the regulation of MXR function and (iv) the importance of the MXR modulators for the assessment of ecotoxicological effects of pollutants.
Multixenobiotic resistance (MXR) ; Multidrug resistance (MDR) ; Clam ; Corbicula fluminea ; P-170 glycoprotein ; Chemosensitizer
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Podaci o izdanju
342 (3-4)
1995.
113-123
objavljeno
1383-5718
1879-3592
10.1016/0165-1218(95)90021-7