Molecular Detection of Ewing Sarcoma Translocation t(11 ; 22) by RT-PCR (CROSBI ID 518081)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Dabić Mirt, Mirela ; Meljanac, Kristina ; Bonevski, Aleksandra ; Seiwerth, Sven
engleski
Molecular Detection of Ewing Sarcoma Translocation t(11 ; 22) by RT-PCR
Objectives: The Ewing sarcoma (ES) family of tumors appears as a bone or soft tissue lesion in a child or young adult and it is characterized with EWS-FLI1 t(11 ; 22)(q24 ; q12) translocation. Our aim was to follow-up patients with detected translocations in parrafin blocks/fresh tissue, peripheral blood and/or bone marrow. Patients and Methods: From October 2003 until January 2006 we received tumor samples paired with blood samples of eleven patients diagnosed with Ewing sarcoma during the treatment according to EURO EWING 99 protocol and also, we received six blood samples and six bone marrow samples collected from patients who finished the treatment. Total RNA was isolated and the EWS-FLI1 fusion transcripts were detected by RT-PCR using primers EWS22.2 Bam and FLI1C that specifically amplify both 125 pb type 1 fusion and 191 pb type 2 variant. Results: Nine paraffin samples were positive for type I EWS-FLI1 fusion of which two didn't survive, and two for type II. Two blood samples were positive, one for type I, and another one for type II fusion in patients who finished the treatment. Conclusion: This specific RT-PCR assay is essential for Ewing sarcoma diagnosis and monitoring minimal residual disease, especially in EWS-FLI1 positive patients who have finished the treatment protocol.
Ewing sarcoma; EWS-FLI1 translocation; RNA; RT-PCR
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Podaci o prilogu
673-x.
2006.
objavljeno
Podaci o matičnoj publikaciji
Virchows Archiv
Heidelberg: Springer
Podaci o skupu
2nd Inter-congress of the European society of pathology
poster
24.05.2006-27.05.2006
Janjina, Grčka