engleski

Enzymatic deacylations of esterified saccharides. II. De-esterifications of radiolabelled O-acylglucopyranosides by mice serum and liver esterases

14-C-labelled methyl 2, 6-di-O-pivaloyl-alpha-D-glucopyranoside was used as a novel substrate for esterases from mouse serum and liver. Stepwise de-esterification was achieved. Kinetic studies were undertaken to compare deacylation rates for the enzymatic de-esterification using mice sera and liver microsomal fractions, respectively. Esterase activities were studied in mice treated with immunostimulating peptidoglycan monomer and comparison made with esterases from untreated mice.

esterases ; peptidoglycan monomer ; pivaloylated glucopyranosides ; 14-C-labelled carbohydrate

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