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Pregled bibliografske jedinice broj: 25049

Root formation in micropropagated shoots of Sequoia sempervirens using Agrobacterium


Mihaljević, Snježana; Katavić, Vesna; Jelaska, Sibila
Root formation in micropropagated shoots of Sequoia sempervirens using Agrobacterium // Plant science, 141 (1999), 73-80 (međunarodna recenzija, članak, znanstveni)


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Naslov
Root formation in micropropagated shoots of Sequoia sempervirens using Agrobacterium

Autori
Mihaljević, Snježana ; Katavić, Vesna ; Jelaska, Sibila

Izvornik
Plant science (0168-9452) 141 (1999); 73-80

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Sequoia sempervirens; Agrobacterium; pRi 8196; pRi A4; rooting callus; transformation

Sažetak
Improved rooting was demonstrated in coast redwood (Sequoia sempervirens (D.Don.) End.) using an Agrobacterium-mediated gene transfer system. Microcloned shoot-tips of redwood (clone CA3) were inoculated with Agrobacterium rhizogenes wild strain 8196 or with the pRi A4 transconjugant strain of Agrobacterium tumefaciens (C58 chromosomal background) at the basal-cut end. Roots were formed in 58-69% of explants, but without a hairy-root phenotype. Rooting frequency and the number of adventitious roots on inoculated shoot-tips were slightly moderated by the bacterial strain, duration of inoculation and explant support system. Growth of excised roots was very slow on hormone-free medium and opine production could not be confirmed. Callus cultures derived from putative transformed roots were established on medium supplemented with 1.13 uM 2, 4-D and 2.22 uM BA. PCR analysis of callus tissues DNA confirmed that integrative transformation had occurred in Sequoia adventitious roots generated after infection with Agrobacterium strains 8196 or A4. This is the first report of transgenic Sequoia tissue using A. rhizogenes-mediated gene transfer.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
119098

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb


Citiraj ovu publikaciju

Mihaljević, Snježana; Katavić, Vesna; Jelaska, Sibila
Root formation in micropropagated shoots of Sequoia sempervirens using Agrobacterium // Plant science, 141 (1999), 73-80 (međunarodna recenzija, članak, znanstveni)
Mihaljević, S., Katavić, V. & Jelaska, S. (1999) Root formation in micropropagated shoots of Sequoia sempervirens using Agrobacterium. Plant science, 141, 73-80.
@article{article, year = {1999}, pages = {73-80}, keywords = {Sequoia sempervirens, Agrobacterium, pRi 8196, pRi A4, rooting callus, transformation}, journal = {Plant science}, volume = {141}, issn = {0168-9452}, title = {Root formation in micropropagated shoots of Sequoia sempervirens using Agrobacterium}, keyword = {Sequoia sempervirens, Agrobacterium, pRi 8196, pRi A4, rooting callus, transformation} }
@article{article, year = {1999}, pages = {73-80}, keywords = {Sequoia sempervirens, Agrobacterium, pRi 8196, pRi A4, rooting callus, transformation}, journal = {Plant science}, volume = {141}, issn = {0168-9452}, title = {Root formation in micropropagated shoots of Sequoia sempervirens using Agrobacterium}, keyword = {Sequoia sempervirens, Agrobacterium, pRi 8196, pRi A4, rooting callus, transformation} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • Biological Abstracts
  • Current Advances in Plant Sciences





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