engleski

Interleukin-10 Gene Genotypes in Sporadic Colon Cancer

Interleukin-10 (IL-10) is an anti-inflammatory and immuno-suppressive cytokine involved in angiogenesis in various cancers. IL-10 production is influenced by single nucleotide polymorphisms (SNPs) in the promoter region of the gene. We examined IL-10 haplotypes consisting of three SNPs -1082(G/A), -819(C/T) and -592(C/A) within the promoter region of the IL-10 gene. IL-10 production is associated with haplotypes that are defined by these SNPs, so that individuals can be genotyped for higher or lower levels IL10 production. These haplotypes might influence the risk for colon cancer development and progression possibly by affecting the efficiency of the antitumour immune response and/or pathways of angiogenesis. A total of 100 sporadic colon cancer (SCC) patients and 100 unrelated cancer-free controls were genotyped for -1082 SNP using real-time PCR TaqMan® ; ; ; , SNP genotyping assay. PCR-RFLP method was used for -819 and -592 SNPs. The most frequent IL-10 genotypes in both groups were medium expressing genotypes ACC/GCC (30, 0% in control and 29, 9% in SCC) and ATA/GCC (22, 9% in control and 24, 7% in SCC). High producing genotype GCC/GCC was detected in 17, 1 % of controls, and 15, 5% of SCC samples. Low producing genotype ACC/ACC was more frequent in controls (20, 0%) when compared to SCC (6, 2%), and low producing genotype ATA/ATA was more frequent in SCC (8, 2%) when compared to the control where it was not detected at all. Combined genotype ACC/ATA was detected in 10, 0% of control and 15, 5% of SCC samples. Our results indicate that some of the specific IL-10 genotypes might be associated with sporadic colon cancer.

IL-10; polymorphisms; sporadic colon cancer

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