Dielectric spectroscopy of genomic DNA solutions (CROSBI ID 517347)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Vuletić, Tomislav ; Dolanski Babić, Sanja ; Tomić, Silvia ; Krča, Sanja ; Ivanković, Dušica ; Griparić, Lorena
engleski
Dielectric spectroscopy of genomic DNA solutions
Transport of electrical signals in complex biosystems, and in particular in the key molecule of DNA is of high interest. The anisotropic structure of DNA implies that the signaling process should be extremely sensitive to the local environment of the double helix. Revealing the dynamical properties of DNA as a function of its aqueous environment is certainly fundamental for macromolecular mechanisms in the cell. Low frequency dielectric spectroscopy was employed, in the frequency range of 100 Hz to 100 MHz. This is a non-destructive tool, which can be used for detecting and quantifying the polarization response of DNA in aqueous environment. A wide concentration range of salmon sperm and calf-thymus Na-DNA solutions in pure water, and in NaCl electrolyte of varying ionic strength was investigated. We discuss the possible assignments of observed relaxation modes taking into account the value and the concentration dependence of the dielectric relaxation parameters obtained from fits to the generalized Debye function. Persistence length is identified along with other characteristic length scales. We also discuss the influence of DNA on its environment, that is, on the effective diffusion constants of ions in solution.
DNA dielectric relaxation; dielectric spectroscopy of biomaterials
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Podaci o prilogu
640-x.
2005.
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objavljeno
Podaci o matičnoj publikaciji
European Biophysical Journal with Biohysics Letters
Watts, Anthony
Heidelberg: Springer
Podaci o skupu
15th IUPAB and 5th EBSA Intrenational Biophysics Congress
poster
27.07.2005-01.09.2005
Montpellier, Francuska