engleski

Downstream targets of nm23-H1: Gene expression profiling of CAL 27 cells using DNA microarray

The human nm23-H1 was discovered as a tumor metastasis suppressor based on its reduced expression in melanoma cell lines with low vs. high metastatic potential. It encodes for one of two subunits of the nucleoside-diphosphate kinase. Besides its role in the maintenance of the cells NTP pool, nm23 plays a key role in different cellular processes. The role of nm23-H1 in these processes still has to be elucidated. Our goal was to identify Nm23-H1 downstream targets by subjecting Nm23-H1 overexpressing CAL 27 cells (oral squamous cell carcinoma) to microarray analysis. The genes with changed expression patterns could be clustered into several groups: TGF signaling pathway, cell adhesion, invasion and motility, proteasome machinery, cell cycle, epithelial structural and related molecules and others. Based on the expression patterns observed we presume that nm23-H1 might have a role in oral squamous cell carcinomas, which should be confirmed by future experiments.

nm23-H1; DNA microarray; oral squamous cell carcinoma

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