engleski

Glycosylation patterns of placental proteins in blighted ovum

Blighted ovum is a form of miscarriage, which involves the absence or disappearance of an embryo very early in pregnancy. Because contemporary understanding of blighted ovum is not yet clear, we have demonstrated the glycosylation patterns of placental proteins in this failure of pregnancy. Olygosaccharide branches were detected by Western-blot method using lectins: SNA and PHA-E, after preliminary separation of proteins by discontinuous SDS-PAG electrophoresis. We have identified much stronger expression of GP74 in blighted ovum than in normal placenta at the beginning of the eleventh week of gestation (with PHA-E). The same result was obtained for GP25 being stronger in blighted ovum at the end of eleventh week (with SNA). The conclusion can be drawn that differences beetwen the blighted ovum and normal placenta, can be found only at the level of quantitative glycoprotein changes, but not at the qualitative level.

Glycoproteins; lectins; blighted ovum

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