engleski

Method for isolation and purification of human genomic DNA

Studies in molecular biotechnology related to gene structure and DNA restriction fragment analysis require isolation of genomic DNA in a form favoring the activity of restriction enzymes, i.e. purified and intact DNA which will not produce nonspecific DNA fragments by casual splitting. Such fragments, as well as other impurities, cause considerable difficulties during genetic experiments. Great variability in digestion of restriction enzymes has just been attributed to the source of DNA and the method of its preparation. A series of procedures for DNA isolation, differing in some seemingly peety details that are, however, very important for both quality and quantity of isolated DNA, have been described so far. The aim of this study was to isolate a restrictible DNA, i.e. an adequately pure and intact DNA for Southern blot analysis of restriction fragments from whole blood samples.

DNA; isolation; purification

Projekt broj 3-01-253, Ministarstvo znanosti i tehnologije RH