engleski

Influence of cysteamine supplementation during in vitro maturation of bovine oocytes on developmental rate and embryo quality

The present study examined the effect of cysteamine during in vitro maturation (IVM) of bovine oocytes on blastocyst development and embryo quality. After maturation in control (0µ M) or cysteamine supplemented medium (100µ M) oocytes were fertilized and cultured for 9 days. The endpoints for evaluation were: Day 2 cleavage rate ; Day 7 blastocyst rate ; Day 9 hatching rate, total cell number and differentiation of the embryonic cells into inner cell mass cells (ICM) and trophectoderm cells (TE) in Day 9 embryos. No beneficial effect regarding to maturation or cleavage rate was related to the addition of cysteamine. However, the addition of cysteamine resulted in significantly higher percentage of morulas and blastocysts on Day 7 (p<0, 001), hatched blastocysts on Day 9 of culture (p<0, 01) and higher total cell number and the number of ICM cells in Day 9 hatched blastocysts (p<0, 05).

cow ; embryo ; IVM ; cysteamine ; embryo development ; ICM

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