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Transvaginal ovum pick-up in the cow: alternative in the production of bovine embryos for tarnsfer.


Getz, Iva; Matković, Mario; Lojkić, Martina; Makek, Zdenko; Bačić, Goran; Cergolj, Marijan
Transvaginal ovum pick-up in the cow: alternative in the production of bovine embryos for tarnsfer. // Veterinarski arhiv, 70S (2000), Supplement; S75-S80 (podatak o recenziji nije dostupan, kongresno priopcenje, znanstveni)


Naslov
Transvaginal ovum pick-up in the cow: alternative in the production of bovine embryos for tarnsfer.

Autori
Getz, Iva ; Matković, Mario ; Lojkić, Martina ; Makek, Zdenko ; Bačić, Goran ; Cergolj, Marijan

Izvornik
Veterinarski arhiv (0372-5480) 70S (2000), Supplement; S75-S80

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
cow; follicle; aspiration; oocyte; production in vitro; embryo

Sažetak
The use of biotechnology, and therefore embryo transfer and manipulation with embryos in vitro, has became an integral part of animal reproduction. In order to further exploit this technique several oocyte aspiration methods for IVF from previously selected living cows are suggested. A method is described for transvaginal ovum pick-up (OPU) in two donor cows, stimulated with FHS/LH (Stimufol , Merial, France) for 3 consecutive days. Aspiration of the follicles was performed on Day 4 and procedure was repeated once a week for three consecutive weeks. The OPU system (Pie Medical, the Netherlands) consists of 3 components: an ultrasound scanner with a sector 5/7, 5 MHz tranducer, an aspiration pump and a needle guidance system. Donor cows were sedated and after additional relaxation by epidural anesthesia, follicles were punctured under echographic control. The number of oocytes (cumulus-oocyte complex = COC) collected using OPU varied from 1 to 6 (2.5 an average) and mean recovery rate was 44.1%. Fertilization rate after 24 hours of oocytes-sperm co-incubation was 53.3%. Culture in vitro in SOFaaBSA media rendered an average of 33.3% morulas and blastocysts of good quality, for ET or cryopreservation.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
053040

Ustanove
Veterinarski fakultet, Zagreb

Profili:

Avatar Url Zdenko Makek (autor)

Avatar Url Goran Bačić (autor)

Avatar Url Mario Matković (autor)

Avatar Url Iva Getz (autor)

Avatar Url Marijan Cergolj (autor)

Avatar Url Martina Lojkić (autor)

Citiraj ovu publikaciju

Getz, Iva; Matković, Mario; Lojkić, Martina; Makek, Zdenko; Bačić, Goran; Cergolj, Marijan
Transvaginal ovum pick-up in the cow: alternative in the production of bovine embryos for tarnsfer. // Veterinarski arhiv, 70S (2000), Supplement; S75-S80 (podatak o recenziji nije dostupan, kongresno priopcenje, znanstveni)
Getz, I., Matković, M., Lojkić, M., Makek, Z., Bačić, G. & Cergolj, M. (2000) Transvaginal ovum pick-up in the cow: alternative in the production of bovine embryos for tarnsfer.. Veterinarski arhiv, 70S (Supplement), S75-S80.
@article{article, year = {2000}, pages = {S75-S80}, keywords = {cow, follicle, aspiration, oocyte, production in vitro, embryo}, journal = {Veterinarski arhiv}, volume = {70S}, number = {Supplement}, issn = {0372-5480}, title = {Transvaginal ovum pick-up in the cow: alternative in the production of bovine embryos for tarnsfer.}, keyword = {cow, follicle, aspiration, oocyte, production in vitro, embryo} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus