engleski

Metallothionein Expression and Tissue Metal Kinetics After Partial Hepatectomy in Mice

To better elucidate previous results showing that partial hepatectomy noticeably changes the tissue content of zinc, calcium, magnesium and iron (II) ions in regenerating liver, thymus and spleen, we report on the correlation of these metal tissue kinetics in these organs with the expression of metallothionein-I+II (MT-I+II) proteins and MT-I mRNA in early postoperative period (1, 2, 6, 12 and 24 hours) after one-third hepatectomy (pHx). The results showed that two hours after pHx the regenerating liver accumulated Zn^2+, Ca^2+, Mg^2+ and Fe^2+ ions while simultaneously decreased the concentration of all these metals in the spleen, and of Zn^2+ in the thymus. On the 24^th hour a new high accumulation of Zn^2+ and Ca^2+ was seen in the regenerating liver, and of Zn^2+, Ca^2+ and Fe^2+ in the spleen. Simultaneously, in hepatic and splenic tissue increased the MT-I mRNA, while in hepatocytes and on several splenic and thymic mononuclear lymphatic cells the increased expression of MT proteins was found in the cytoplasm and nuclei. The areas expressing MTs in regenerating liver inversely correlated with those containing apoptotic cells, suggesting that these proteins participate in tissue restoration through reduction or increase of metal ions after injury to the liver.

zinc; iron; calcium; magnesium; ICP spectroscopy; atomic absorption; immunohistochemistry; metallothionein; liver regeneration; thymus; spleen

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