engleski

Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria

The available data concerning rapid identification of fish bacteria via commercial phenotypic tests demonstrate that there is no agreement regarding the choice of selection of the tests. However, API 20E, an identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods developed for clinical specimens, seems to be increasingly used for the identification of fish pathogens. In this work, adaptation of API 20E for fish bacterial isolates and its distinctiveness for fish bacteria was assessed. For some bacterial fish isolates, their identification must be regarded as only presumptive. Some strains are wrongly identified because they are not included in the database of API 20E system. For comparable results, API 20E has to be adapted for bacterial fish isolates, and there should be a consensus among the fish health professionals regarding the incubation temperature and the duration of the incubation time before performing readings. API 20E reactions should be compared with the diagnostic schemes based on reactions in conventional phenotypic tests. Due to their significance for fish health and impact on the aquaculture, and because of the need for their rapid identification, some important fish bacteria should be included in the API 20E system, such as Yersinia ruckeri, Edwardsiella ictaluri, Vibrio anguillarum.

API 20E; bacteria; diagnostics; fish

nije evidentirano