engleski

Acid phosphatase activity in the liver of mice with transplanted leukaemia

The activity of acid phosphatase, used as a lysosomal marker enzyme, was studied in the livers of mice 2, 4, 6, 8 and 10 days after inoculation of a strain-specific lymphatic leukaemia. Total and unsedimentable activities of the enzyme were assayed in whole homogenates of the liver, whereas a lysosome-rich preparation obtained by gradient centrifugation was used for measurements of total and free activities. Unsedimentable and free activities increased 4 days after inoculation of leukaemic cells. Thereafter, the unsedimentable activity remained high until death 10 days post inoculation, while the free activity decreased to the control values on day 6 and again increased on days 8 and 10. On the other hand, total activity of the enzyme, both in homogenates and in the lysosome-rich preparations, decreased slightly but progressively until death. These alterations were interpreted as indicating an in vivo release of acid phosphatase in the liver as a response of liver reticuloendothelium against leukaemic cells, and progressive infiltration of this organ with lysosome-poor leukaemic cells.

acid phosphatase; leukaemia

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