Kinetic model of tocopherol-mediated peroxidation (CROSBI ID 468984)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Herak, Janko ; Krilov, Dubravka ; Stojanović, Nataša
engleski
Kinetic model of tocopherol-mediated peroxidation
Slow oxidation of low-density lipoprotein (LDL) is characterized by tocopherol-mediated peroxidation. Our experiments, performed under the conditions of very mild oxidation in a closed system (with the presence of EDTA), gave the evidence that in this process alpha-tocopherol (TH) was not used to a detectable level in several days. At the same time, after about one day, oxygen was consumed and peroxides were fromed. The further phase of the process was characterized by the reduction of peroxides and the generation of free radicals, trapped predominantly on the apoprotein. Bases on these experimental observations, a kinetic model of tocopherol-mediated oxidation of LDL has been developed. The initiation phase of oxidation was modelled by processes that are associated with the switching of the chelated metal ions between the oxidized and reduced states, and simultaneous switching of TH between the reduced and oxidized states. These processes include homolytic cleavage of preexisting lipid hydroperoxides (LOOH), and subsequent formation of lipid hydroxides (LOH) and destruction of co-antioxidants. In the second phase The lipid (L.) and lipid peroxy radicals (LOO.) serve as chain propagating species, together with the oscillation of tocopherol between TH and T. states. In the final phase, after consumption of oxygen, lipid peroxides decompose and free radicals are formed, which react with the protein moiety and ultimately terminate the process.
LDL oxidation; Heterogeneous kinetics; Compartments; Probabilistic kinetic model
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Podaci o prilogu
87-87-x.
1998.
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objavljeno
Podaci o matičnoj publikaciji
Book of abstracts, Annual Meeting of Croatian Biochemists
Glavaš-Obrovac, Ljubica
Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu
Podaci o skupu
Godišnji sastanak hrvatskih biokemičara
poster
17.09.1998-20.09.1998
Hrvatska