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Slow oxidation of high density lipoproteins as studied by EPR spectroscopy (CROSBI ID 121206)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Stojanović, Nataša ; Krilov, Dubravka ; Herak, Janko N. Slow oxidation of high density lipoproteins as studied by EPR spectroscopy // Free radical research, 40 (2006), 2; 135-140-x

Podaci o odgovornosti

Stojanović, Nataša ; Krilov, Dubravka ; Herak, Janko N.

engleski

Slow oxidation of high density lipoproteins as studied by EPR spectroscopy

There is relatively little information on the role of HDL oxidation in atherogenesis although there are indications that oxidation might affect atheroprotective activities of HDL. Recently we reported the study on LDL oxidation initiated and sustained by traces of the transition metal ions under conditions which favor slow oxidation. Here we report the results of the analogous study on the oxidation of the two HDL subclasses. The oxidation process was monitored by measuring the time dependence of oxygen consumption and concentration of the spin-trapped free radicals using EPR spectroscopy. In both HDL2 and HDL3 subclasses, the dependence of the oxidation process on the copper/lipoprotein molar ratio is different from that in LDL dispersions. Comparison of the kinetic profiles of HDL2 and HDL3 oxidation revealed that under all studied experimental conditions HDL2 was more susceptible to copper-induced oxidation than HDL3.

high density lipoprotein; slow oxidation; copper ions; EPR spectroscopy

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Podaci o izdanju

40 (2)

2006.

135-140-x

objavljeno

1071-5762

Povezanost rada

Fizika

Indeksiranost