Extracellular metalloendopeptidase of Streptomyces rimosus (CROSBI ID 121153)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Vitale, Ljubinka ; Vukelić, Bojana ; Križaj, Igor
engleski
Extracellular metalloendopeptidase of Streptomyces rimosus
Metalloendopeptidase was isolated from Streptomyces rimosus culture filtrates in a homogeneous form. It was determined to be a 15 kDa basic protein, most active around pH 7.5, and susceptible to inhibition by chelating agents, N-bromosuccinimide, thiorphan, and 10^-^4M zinc. The enzyme was highly specific for phenylalanine at the N-side of endopeptide bonds. Determination of amino acid sequence of the enzyme^, s NH_2-part allowed the recognition of its structure homology with several Streptomyces species. The data contribute to the definition of M7 family of metalloendopeptidases in streptomycetes.
Metalloendopeptidase ; Specificity ; Amino acid sequence ; Streptomyces rimosus
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
185 (3)
2006.
183-191
objavljeno
0302-8933
1432-072X
10.1007/s00203-006-0084-x