engleski

Characterization of a Noncyst-forming Isolate of Trichinella from a Wild Boar in Yugoslavia

An isolate of Trichinella obtained from a wild boar in Yugoslavia did not form cysts in the musculature of its natural host. Subsequent inoculation into experimental hosts demonstrated that some larvae became encysted only after extended time periods, whereas other remained unencapsulated. Histological staining of larvae in the musculature demonstrated no deposition of collagen typically seen for Trichinella spiralis spiralis, Trichinella spiralis nativa, or Trichinella spiralis nelsoni. The Yugoslavian isolate, given the name of Zagreb isolate after the University whee was first studied, had low infectivity for pigs and mice. Isozyme analysis demonstrated greater homology with T. s. nelsoni than with other subspecies of Trichinella. Restriction fragment length polymorphisms and dot blot analyses futrher demonstrated the distinctive nature of this isolate. These results suggest that lack of cyst formation might be characteristic of isolate other than those designated Trichinella pseudospiralis and that this character might be important in the classification of Trichinella.

Trichinella; Zagreb isolate; pigs

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