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Solid phase extraction and HPLC determination of veterinary pharmaceuticals in wastewater

This work focuses on the application of SPE-HPLC analysis of important veterinary pharmaceuticals from different classes in highly complex wastewater matrix. The pharmaceutical investigated included three sulfonamides (sulfamethazine, sulfadiazine and sulfaguanidine), a sulfonamide synergist (trimethoprim), a tetracycline (oxytetracycline), a fluoroquinolone (enrofloxacine) and a  -lactame (penicilline G / procaine). The method involves pre-concentration and clean-up by soild phase extraction (SPE) using Oasis HLB extraction catridges. Final analysis of the selected pharamceutical compounds was carried out by high-performance liquid chromatography (HPLC) coupled with diode array detector (DAD). Recoveries were ranged from 68.3 to 97.9 % with relative standard deviation below 8.4 %. Only for sulfaguanidine low recovery was obtained. Limits of quantification were in the range 1.5-100  g/L depending on pharmaceutical. The described method was applied to the determination of pharmaceuticals in wastewater samples from pharmaceutical industry.

pharmaceuticals ; wastewater ; high-performance liquid chromatography ; diode array detection ; soild-phase extraction

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