Sporulation in diploid transformants of Saccharomyces cerevisiae obtained by illegitimate integration of single-stranded DNA (CROSBI ID 82625)
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Gjuračić, Krešimir ; Zgaga, Zoran
engleski
Sporulation in diploid transformants of Saccharomyces cerevisiae obtained by illegitimate integration of single-stranded DNA
DNA sequence analysis and chromosomal polymorphism of natural and industrial yeast strains reveal important role of illegitimate recombination in the evolution of the yeast genome. The resulting genetic changes can be brought to the homozygous state by sporulation and mating between haploid progeny. In this work we compared sporulation of diploid transformants obtained by illegitimate integration of homologous single-stranded (ss) DNA and transformants obtained with double-stranded (ds) plasmid that replicates autonomously in the yeast cell. The transformants that contained exogenous DNA integrated by illegitimate recombination had lower efficiency of sporulation, but the spore viability was decreased from 80% to 65% only among preselected fraction of transformants. Some of the surviving haploids contained transforming DNA integrated in their genome. Possible mechanisms that led to the decrease in sporulation efficiency and spore viability in transformants obtained by illegitimate integration of foreign DNA into yeast genome are discussed.
Saccharomyces cerevisiae; illegitimate recombination; sporulation
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