engleski

Preliminary studies on bacterial diversity of cultured bluefin tuna Thunnus thynnus from the Adriatic Sea

Information on the bacterial population associated with cultured tuna are scarce, particularly for bluefin tuna (Thunnus thynnus). The aim of this work was to investigate the initial microflora of bluefin tuna in the Adriatic Sea. Four bluefin tunas weighing 6.5 to 15.0 kg were obtained from tuna farm in the Middle Adriatic. Fish samples were taken in spring and autumn from 2003 to 2005. Samples for bacterial isolation were collected from skin, gills, spleen, liver and kidney, and cultured on Tryptic soy agar (BBL) supplemented with 1 % NaCl. Isolated strains were further characterized using the API kits (bio Mérieux, France) and identified by the APILAB Plus identification software (bio Mérieux, France). Gram-negative bacteria from genera Moraxella, Pasteurella, Brevundimonas, Weeksella, Klebsiella and Pseudomonas dominated the microflora on examined tuna, whereas Staphylococcus was the only one Gram- positive isolated genus.

bluefin tuna; initial microflora; Middle Adriatic

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