Homologous Recombination and DNA Repair in recBC sbcBC Mutants of Escherichia coli: Effects of a recG Mutation (CROSBI ID 514304)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Zahradka, Davor ; Buljubašić, Maja ; Zahradka, Ksenija ; Petranović, Mirjana
engleski
Homologous Recombination and DNA Repair in recBC sbcBC Mutants of Escherichia coli: Effects of a recG Mutation
RecG protein is a DNA helicase that promotes DNA strand exchange and heteroduplex extension during homologous recombination in Escherichia coli. Cells mutated in the recG gene show moderate recombination deficiency and increased UV-sensitivity compared to the wild type cells. We have studied the effects of a recG mutation on recombinational DNA repair after exposure to UV-irradiation, on conjugal recombination, and on cell morphology in two types of recBC sbcBC mutants of E. coli. In one mutant, the sbcB gene was inactivated by point mutation (sbcB15), while in other it was completely deleted (delsbcB). We found that introduction of the recG mutation in a recBC sbcB15 sbcC background strongly reduced the efficiency of recombination and recombinational DNA repair, and caused severe defects in chromosome segregation and cell division. On the contrary, in recBC delsbcB sbcC background, the recG mutation had relatively mild effect on both recombination processes and cell morphology. We inferred that the sbcB15 mutation modifies recombinational process in a way that the process becomes more dependent on the RecG protein. It is possible that the mutant SbcB15 protein binds to recombinogenic 3' DNA ends and interferes with the processing of recombination intermediates in the absence of RecG protein.
homologous recombination; DNA repair; recBC sbcBC mutants; recG mutation
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Podaci o prilogu
174-175.
2005.
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objavljeno
Podaci o matičnoj publikaciji
Acta microbiologica et immunologica Hungarica
Marialigeti, K. ; Sipos, R.
Budimpešta: Akadémiai Kiadó
1217-8950
Podaci o skupu
Central European Forum for Microbiology (1 ; 2005)
poster
26.10.2005-28.10.2005
Budimpešta, Mađarska