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Pregled bibliografske jedinice broj: 228360

Enumeration of haemagglutinin-specific CD8+ T cells after influenza vaccination using MHC class I peptide tetramers


Kosor, Ela; Gagro, Alenka; Draženović, Vladimir; Kuzman, Ilija; Jeren, Tatjana; Čečuk-Jeličić, Esma; Kerhin-Brkljačić, Vesna; Gjenero-Margan, Ira; Kaić, Bernard; Rakušić, Snježana et al.
Enumeration of haemagglutinin-specific CD8+ T cells after influenza vaccination using MHC class I peptide tetramers // Scandinavian journal of immunology, 67 (2007), 1; 86-94 doi:0.1111/j.1365-3083.2007.02042.x (međunarodna recenzija, članak, znanstveni)


Naslov
Enumeration of haemagglutinin-specific CD8+ T cells after influenza vaccination using MHC class I peptide tetramers

Autori
Kosor, Ela ; Gagro, Alenka ; Draženović, Vladimir ; Kuzman, Ilija ; Jeren, Tatjana ; Čečuk-Jeličić, Esma ; Kerhin-Brkljačić, Vesna ; Gjenero-Margan, Ira ; Kaić, Bernard ; Rakušić, Snježana ; Sabioncello, Ante ; Markotić, Alemka ; Rabatić, Sabina ; Mlinarić-Galinović, Gordana ; Dekaris, Dragan

Izvornik
Scandinavian journal of immunology (0300-9475) 67 (2007), 1; 86-94

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Influenza; vaccine; MHC class I tetramers; CD8+ T cells

Sažetak
With emergence of MHC class I tetramers loaded with CD8+ T-cell viral epitopes, it is possible to study virus-specific CD8 cells in humans during infection and after vaccination. MHC class I tetramers was used to detect the frequency of haemagglutinin (HA)-specific T cells in 26 healthy influenza-vaccinated humans. Peripheral blood was collected before, and 7, 14 and 28 days after vaccination. Four-colour flow cytometry was used for monitoring of vaccine induced T-cell response. In 15 donors, two- to fivefold increase in frequency of HA-specific T cells was observed 7 days after vaccination. In addition, in 12 of these donors, this increase was accompanied with fourfold increase of H1N1 antibody titre. The increase in frequency of HA-specific CD8+/IFN-gamma+ cells was low and peaked 28 days after vaccination in three of the six donors tested. Frequencies of HA-specific CD8+ T cells and antibody titre returned to prevaccination values 1 year after vaccination. Subunit influenza vaccines have the ability to induce HA-specific CD8+ cells. As the immune response to this vaccine decreased significantly after 1 year, our results confirm the importance of annual immunization for adequate protection.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

Napomena
Dio istraživanja opisanih u radu financiran je i sredstvima projekta TP-01/0021-05 (voditelj Alenka Gagro).



POVEZANOST RADA


Projekt / tema
021-0212432-2434 - Psihološki faktori u modulaciji imunoreaktivnosti (Ante Sabioncello, )
072-1080229-0337 - Modulacija funkcije ljudskih regulacijskih T-limfocita (Alenka Gagro, )
143-1430115-0103 - Imunoreakcije na hantaviruse i leptospire (Alemka Markotić, )

Ustanove
Hrvatski zavod za javno zdravstvo,
Imunološki zavod d.d.,
Klinika za infektivne bolesti "Dr Fran Mihaljević",
Klinički bolnički centar Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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