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Pregled bibliografske jedinice broj: 228215

Nova metoda za određivanje glikozilacije proteina


Gornik, Olga; Flogel, Mirna; Lauc, Gordan
Nova metoda za određivanje glikozilacije proteina // Treći hrvatski kongres farmacije s međunarodnim sudjelovanjem, Knjiga sažetaka / Zorc, Branka (ur.).
Zagreb: Farmaceutsko-biokemijski fakultet, 2005. (poster, nije recenziran, sažetak, znanstveni)


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Naslov
Nova metoda za određivanje glikozilacije proteina
(NEW METHOD FOR ANALYSING PROTEIN GLYCOSYLATION)

Autori
Gornik, Olga ; Flogel, Mirna ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Treći hrvatski kongres farmacije s međunarodnim sudjelovanjem, Knjiga sažetaka / Zorc, Branka - Zagreb : Farmaceutsko-biokemijski fakultet, 2005

Skup
Treći hrvatski kongres farmacije s međunarodnim sudjelovanjem

Mjesto i datum
Cavtat, Hrvatska, 27-30.04.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
glikoproteini; ELISA
(glicoproteins; ELISA)

Sažetak
Glycans are the most abundant and most diverse biopolymers in nature and participate in many crucial biological processes. They are involved in genesis, development and processing of many diseases, and many diseases are associated with changes in glycan structures. Because of all that glycans are now in the focus of modern medicine, biotechnology and pharmacy through the improvement of diagnostics, disease monitoring and development of biotherapeutics that are mainly (glyco)proteins. Glycan structures are very complex and methods to analyze them are very demanding and time-consuming, thus the availability of simple techniques for the analysis of oligosaccharide structures and their changes could be a very helpful tool. Aiming to establish such a method, we are developing a lectin based ELISA method for the analysis of transferrin sialylation. Transferrin is a serum glycoprotein that has two N-linked oligosaccharide chains consisting of bi- or three- antennary structures, each possibly terminating in sialic acid. SNA (Sambucus nigra agglutinin) lectin specifically recognizes Neu5Ac( 2-6)Gal/GalNAc sequence present on transferrin and this lectin was used to develop the assay. Our method allows us to determine the level of sialic acid on the protein tested, having the protein mixture (serum) as a sample by using immobilized anti-transferrin antibody and to compare the results acquired on different multiwell plates by using standard curve of pure transferrin on each plate. If glycosylation results of differently sialylated controls obtained by lectin ELISA are put in correlation with their HPAE-PAD analysis even more detailed information could be obtained. Since many studies suggested changes of transferrin glycosylation in various diseases, this is a very useful diagnostic method. Similar method could be developed for glycosylation analysis of other proteins. This would be particularly important for glycoprotein therapeutics, since glycosylation changes can significantly influence function of these molecules.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
0006611

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb

Citiraj ovu publikaciju

Gornik, Olga; Flogel, Mirna; Lauc, Gordan
Nova metoda za određivanje glikozilacije proteina // Treći hrvatski kongres farmacije s međunarodnim sudjelovanjem, Knjiga sažetaka / Zorc, Branka (ur.).
Zagreb: Farmaceutsko-biokemijski fakultet, 2005. (poster, nije recenziran, sažetak, znanstveni)
Gornik, O., Flogel, M. & Lauc, G. (2005) Nova metoda za određivanje glikozilacije proteina. U: Zorc, B. (ur.)Treći hrvatski kongres farmacije s međunarodnim sudjelovanjem, Knjiga sažetaka.
@article{article, editor = {Zorc, B.}, year = {2005}, pages = {127}, keywords = {glicoproteins, ELISA}, title = {NEW METHOD FOR ANALYSING PROTEIN GLYCOSYLATION}, keyword = {glicoproteins, ELISA}, publisher = {Farmaceutsko-biokemijski fakultet}, publisherplace = {Cavtat, Hrvatska} }