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The synthetic kappa-opioid agonist (-)U50,488 does not affect calcium transport into R1.1 mouse thymoma cell line

In this paper, the effect of the synthetic kappa-opioid agonist (-)U50,488 on 45Ca2+ transport into R1.1 mouse thymoma cells is presented. This thymoma cell line expresses selectively the kappa-opioid class of receptors. 45Ca2+ transport into R1.1 cells was not affected by the kappa-opioid agonist (-)U50,488 (10-10 M - 10-4 M) alone, or in the presence of the plant lectins: PHA (250 microg/ml) and Con A (800 microg/ml), after a 60-min treatment. The plant lectins PHA and Con A stimulated 45Ca2+ transport into R1.1 cells, in high concentrations (100-800 microg/ml) and (200-1000 microg/ml) respectively, after a 60-min treatment. Thus, 45Ca2+ transport was not affected in R1.1 cells by the k-opioid agonist (-) U50,488 alone, or in the presence of mitogens after a 60-min treatment. This negative result does not indicate the lack of calcium channels on R1.1 cells, since the plant lectins PHA and Con A were able to stimulate 45Ca2+ transport into R1.1 cells. In this paper, the effect of the synthetic k-opioid agonist (-)U50,488 on 45Ca2+ transport into R1.1 mouse thymoma cell line is presented. This thymoma cell line expresses the k-opiod class of receptors. 45Ca2+ transport into R1.1 cells was not affected by the k-opioid (-)U50,488 (10-10M - 10-4M) alone, or in the presence of the plant lectins: PHA (250 microg/microL) and Con A (800 microg/microL) after a 60 min treatment. The plant lectins PHA and Con A stimulated 45Ca2+ transport into R1.1 cells in high concentrations (100-800 microg/microL) and (200-1000 microg/microL) respectively, after a 60 min treatment. Thus, 45Ca2+ transport was not affected in R1.1 cells by the k-opioid agonist (-)U50,488 alone, or in the presence of mitogens after a 60 min treatment. This negative result does not indicate the lack of calcium channels on R1.1 cells, since the plant lectins PHA and ConA were able to stimulate 45Ca2+ transport into R1.1

calcium transport; kappa-opioid; mouse thymoma

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