engleski

Rapid TLC method for simultaneous fumonisin detection and isolation without derivatization

Fumonisins, members of a new class of sphinganine analog mycotoxins, produced by Fusarium moniliforme and other Fusarium species, widely infect corn. Fumonisins are saturated long chain compounds that have no UV apsorbing chromophore, nor do they fluorescence. They contain a free primary amine and multiple freecarboxyl groups so minor changes in pH or ionic strenght in media, can remarkably affect on isolation and chromatographic behavior. In order to overcome these limitations, in thi study we present a different, artless and simple approach. After the biosynthesis of an F. moniliforme culture in solid and liquid substrate media. acetonitrile/water mixture efficiently separate fumonisins. The water soluble phase, adjusted to pH 8-9 with NaHCO3, is then shaking with chloroform for subsequent purification. Water-soluble phase is concentrated in vacum by liophilisation. Solutions were spotted on preperative GF254 silica plates and develop with acetonitrile/toluene/water (93:3:2). Detection of FB1 is posible directly through florescence quenching in short UV light as bright blue zones.Confirmation of FB1 presence was made by GC/MS method after hydrolisis and derivatisation with TMS reagent. In EIMS of the TMS derivates of the C-22 aminotetraols from hydrolisis products of FB1 backbones, the protonated molecular ions for the penta-TMS derivates and for tetra- TMS derivates as well as abundant fragments from cleavage between C-14 and C-15 were presented. The results indicated that this simple TLC method may be convinient in folloving the isolation of metabolites from extracts of Fusarium cultures

Fumonisins; TLC method Fusarium monilforme

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