Naslov
Measurement of salivary insulin-like growth factor-I in acromegaly: Comparison with serum insulin-like growth factor-I and growth hormone concentrations

Autori
Halimi, Setar ; Tepavčević, Danilo ; Suchanek, Ernest ; Giljević, Zlatko ; Plavšić, Vesna ; Koršić, Mirko

Izvornik
European Journal of Clinical Chemistry and Clinical Biochemistry (1434-6621) 32 (1994); 705-707

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Salivary insulin-like growth factor-I; Acromegaly; Growth hormone

Sažetak
The aim of this study was to establish the concentration of insulin-like growth factor-I (IGF-I) in saliva of acromegalic patients, and to compare it with the basal levels of serum IGF-I and growth hormone. IGF-I was determined in extracted serum or neat saliva by a disequilibrium RIA using antibodies and iodinated ligand from Amersham and WHO 87/518 as standard. The detection limit of the assay was 0.5 ug/L, and the intra- and inter-assay coefficients of variations were 7.9% and 15%, respectively. Our study included 13 healthy adult individuals and 17 acromegalics. Compared with healthy adult subjects, acromegalics had singnificantly higher salivary IGF-I concentrations (mean +/- SEM 5.4 +/- 2, 64 vs. 10.5 +/- 5, 69 ug/L ; p<0.01), as well as serum IGF-I (176 +/- 42.9 vs. 520 +/- 98.8 ug/L ; p<0.0001) and somatotropin levels (1.2 +/- 1.02 vs. 15.4 +/- 9.89 ug/L ; p<0.0001). However, 47.1% patients (8 out of 17) with active acromegaly had salivary IGF-I concentrations within the normal range. Serum IGF-I and somatotropin concentrations were found to follow more closely the disease activity after adenomectomy, compared with the concentrations of salivary IGF-I. These results suggest that the IGF-I levels in serum and saliva are somatotropin-dependent. According to our results, measurement of IGF-I in saliva cannot be considered as an additional measure for evaluation of the disease activity in acromegaly, being less reliable than the determination of IGF-I and somatotropin in serum.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA