engleski

Metallo-beta -lactamase production and susceptibility to antibiotics of Pseudomonas aeruginosa isolates from Zagreb

The aim of this study was to determine the susceptibilities to wide range of antibiotics of P. aeruginosa isolates with reduced susceptibility to either imipenem or meropenem and the prevalence of metallo-beta lactamases (MBLs)produced by these isolates. 100 P. aeruginosa isolates with reduced susceptibility to carbapenems were collected in three hospitals in Zagreb during 2002-2004. None of the antibiotic tested in this study(meropenem, imipenem, ciprofloxacin, piperacillin, piperacillin combined with tazobactam, ceftazidime, cefoperazone, gentamicin) should be used as treatment of choice for P.aeruginosa strains with reduced susceptibility to carbapenems due to high percentage of resistance.Combined-disk test has shown to be the most sensitive in detection of MBLs, followed double -disk test. E-test gave the lowest rate of positive results. Specificity of all three test was 100%. As previously observed by other authors, E test detects MBLs only in strains with high level enzyme production and this can be an explanation for imperfect correlation between phenotypic tests for MBLs.

metallo-beta-lactamase; antibiotic; susceptibility; Pseudomonas aeruginosa

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