Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 220245

Analysis of Leulocyte Subpopulations from the Lungs of Mice Infected with Legionella longbeachae


Gobin, Ivana; Trobonjača, Zlatko; Begić, Gabrijela; Hlača-Caput Tamara; Abu Kwaik, Yousef; Dorić, Miljenko; Šuša, Milorad
Analysis of Leulocyte Subpopulations from the Lungs of Mice Infected with Legionella longbeachae // Annual Meeting of Croatian Immunological Society. Book of Abstracts / Jonjić, Stipan ; Dekaris, Dragan ; Polić, Bojan ; Gagro, Alenka ; Vitale, Branko ; Malenica, Branko ; Rabatić, Sabina ; Barac-latas, Vesna ; Trobonjača, Zlatko ; Božić, Frane ; Bubić, Ivan (ur.).
Rijeka: Croatian immunological society, 2005. str. 37-37 (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 220245 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Analysis of Leulocyte Subpopulations from the Lungs of Mice Infected with Legionella longbeachae

Autori
Gobin, Ivana ; Trobonjača, Zlatko ; Begić, Gabrijela ; Hlača-Caput Tamara ; Abu Kwaik, Yousef ; Dorić, Miljenko ; Šuša, Milorad

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual Meeting of Croatian Immunological Society. Book of Abstracts / Jonjić, Stipan ; Dekaris, Dragan ; Polić, Bojan ; Gagro, Alenka ; Vitale, Branko ; Malenica, Branko ; Rabatić, Sabina ; Barac-latas, Vesna ; Trobonjača, Zlatko ; Božić, Frane ; Bubić, Ivan - Rijeka : Croatian immunological society, 2005, 37-37

Skup
Annual Meeting of Croatian Immunological Society

Mjesto i datum
Božava, Hrvatska, 29.9-2.10.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
legionella longbeachae; leukocyte subpopulations; infection of mice

Sažetak
Legionella longbeachae serogroup 1 is an uncommon pathogen in most parts of the world but causes up to half of all cases of legionellosis in many regions of Australia. Previous studies showed that L. longbeachae sg 1 in a mouse model of lung infection has a high infectivity potential (LD50 ~104 CFU). Mice infected intratracheally with a sublethal dose (103 CFU) developed acute bronchopneumonia, but survived. We analyzed leukocyte populations attracted in the lungs of C57Bl/6 mice during the course of a sublethal infection with L. longbeachae sg 1. C57Bl/6 mice, were intratracheally inoculated with 103 CFU of L. longbeachae sg 1 (strain D4968). At different time points after infection leukocytes, obtained from lungs of infected animals were phenotypised using monoclonal antibody specific for the following leukocyte surface antigens: α -CD11b, α -CD80, α -CD86, α -CD3, α -CD4, α -CD8, α -NK1.1, α -CD69, α -CD45RB, and α -GR-1. Three colour immunofluorescence analysis was performed by BD FACS Calibur™ flow cytometer using CellQuest™ software. We detected increase of CD11b+ cells in the lungs, 3 days post infection. Two populations of CD11b+ cells were found: CD11b+bright and CD11b+dimm cells (80% and 20% respectively). CD11b+bright cells were simultaneously positive for the granulocyte marker GR-1. An accumulation of CD3+ cells and an inversion of the CD4/CD8 ratio in favor of CD8+ T lymphocytes was detected in the lungs of infected mice. Intratracheal infection of C57Bl/6 mice with L. longbeachae sg 1 induced bronchopneumonia that was accompanied by a massive recruitment of inflammatory cells in the lungs. Majority of the recruited cells expresed the CD11b antigen and coexpressed the granulocyte specific GR-1 antigen. 2 to 3 days after infection the lungs were infiltrated with T lymphocytes, particularly with the CD8+ subset.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
0062064

Ustanove
Medicinski fakultet, Rijeka

Profili:

Avatar Url Ivana Gobin (autor)

Avatar Url Miljenko Dorić (autor)

Avatar Url Milorad Šuša (autor)

Avatar Url Zlatko Trobonjača (autor)

Citiraj ovu publikaciju

Gobin, Ivana; Trobonjača, Zlatko; Begić, Gabrijela; Hlača-Caput Tamara; Abu Kwaik, Yousef; Dorić, Miljenko; Šuša, Milorad
Analysis of Leulocyte Subpopulations from the Lungs of Mice Infected with Legionella longbeachae // Annual Meeting of Croatian Immunological Society. Book of Abstracts / Jonjić, Stipan ; Dekaris, Dragan ; Polić, Bojan ; Gagro, Alenka ; Vitale, Branko ; Malenica, Branko ; Rabatić, Sabina ; Barac-latas, Vesna ; Trobonjača, Zlatko ; Božić, Frane ; Bubić, Ivan (ur.).
Rijeka: Croatian immunological society, 2005. str. 37-37 (poster, nije recenziran, sažetak, znanstveni)
Gobin, I., Trobonjača, Z., Begić, G., Hlača-Caput Tamara, Abu Kwaik, Y., Dorić, M. & Šuša, M. (2005) Analysis of Leulocyte Subpopulations from the Lungs of Mice Infected with Legionella longbeachae. U: Jonjić, S., Dekaris, D., Polić, B., Gagro, A., Vitale, B., Malenica, B., Rabatić, S., Barac-latas, V., Trobonjača, Z., Božić, F. & Bubić, I. (ur.)Annual Meeting of Croatian Immunological Society. Book of Abstracts.
@article{article, year = {2005}, pages = {37-37}, keywords = {legionella longbeachae, leukocyte subpopulations, infection of mice}, title = {Analysis of Leulocyte Subpopulations from the Lungs of Mice Infected with Legionella longbeachae}, keyword = {legionella longbeachae, leukocyte subpopulations, infection of mice}, publisher = {Croatian immunological society}, publisherplace = {Bo\v{z}ava, Hrvatska} }




Contrast
Increase Font
Decrease Font
Dyslexic Font