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Pregled bibliografske jedinice broj: 219127

Analysis of gangliosides directly from thin-layer chromatography plates by infrared matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry with a glycerol matrix.


Dreisewerd, Klaus; Muething, Johannes; Rohlfing, Andreas; Meisen, Iris; Vukelić, Željka; Peter-Katalinić, Jasna; Hillenkamp, Franz; Berkenkamp, Stephan
Analysis of gangliosides directly from thin-layer chromatography plates by infrared matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry with a glycerol matrix. // Analytical chemistry, 77 (2005), 13; 4098-4107 (međunarodna recenzija, članak, znanstveni)


Naslov
Analysis of gangliosides directly from thin-layer chromatography plates by infrared matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry with a glycerol matrix.

Autori
Dreisewerd, Klaus ; Muething, Johannes ; Rohlfing, Andreas ; Meisen, Iris ; Vukelić, Željka ; Peter-Katalinić, Jasna ; Hillenkamp, Franz ; Berkenkamp, Stephan

Izvornik
Analytical chemistry (0003-2700) 77 (2005), 13; 4098-4107

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Gangliosides; thin-layer chromatography; MALDI-MS; glycerol matrix

Sažetak
A novel method is presented for direct coupling of high-performance thin-layer chromatography (HPTLC) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the analysis of biomolecules. A first key feature is the use of a liquid matrix (glycerol), which provides a homogeneous wetting of the silica gel and a simple and fast MALDI preparation protocol. A second is the use of an Er:YAG infrared laser, which ablates layers of approximately 10-microm thickness of analyte-loaded silica gel and provides a soft desorption/ionization of even very labile analyte molecules. The orthogonal time-of-flight mass spectrometer employed in this study, finally provides a high accuracy of the mass determination, which is independent of any irregularity of the silica gel surface. The analytical potential of the method is demonstrated by the compositional mapping of a native GM3 (II(3)-alpha-Neu5Ac-LacCer) ganglioside mixture from cultured Chinese hamster ovary cells. The analysis is characterized by a high relative sensitivity, allowing the simultaneous detection of various major and minor GM3 species directly from individual HPTLC analyte bands. The lateral resolution of the direct HPTLC-MALDI-MS analysis is defined by the laser focus diameter of currently approximately 200 microm. This allows one to determine mobility profiles of individual species with a higher resolution than by reading off the chromatogram by optical absorption. The fluorescent dye primuline was, furthermore, successfully tested as a nondestructive, MALDI-compatible staining agent.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
0108120

Ustanove
Medicinski fakultet, Zagreb

Autor s matičnim brojem:
Željka Vukelić, (241834)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE