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Pregled bibliografske jedinice broj: 218994

Screening and sequencing of complex sialylated and sulfated glycosphingolipid mixtures by negative ion electrospray Fourier transform ion cyclotron resonance mass spectrometry.


Vukelić, Željka; Zamfir, Alina; Bindila, Laura; Froesch, Martin; Peter-Katalinić, Jasna; Usuki, Seigo; Yu, Robert K.
Screening and sequencing of complex sialylated and sulfated glycosphingolipid mixtures by negative ion electrospray Fourier transform ion cyclotron resonance mass spectrometry. // Journal of the American Society for Mass Spectrometry, 16 (2005), 4; 571-580 doi:10.1016/j.jasms.2005.01.013 (međunarodna recenzija, članak, znanstveni)


Naslov
Screening and sequencing of complex sialylated and sulfated glycosphingolipid mixtures by negative ion electrospray Fourier transform ion cyclotron resonance mass spectrometry.

Autori
Vukelić, Željka ; Zamfir, Alina ; Bindila, Laura ; Froesch, Martin ; Peter-Katalinić, Jasna ; Usuki, Seigo ; Yu, Robert K.

Izvornik
Journal of the American Society for Mass Spectrometry (1044-0305) 16 (2005), 4; 571-580

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Complex glycosphingolipid mixtures; negative ion electrospray Fourier transform ion cyclotron resonance mass spectrometry

Sažetak
A protocol for negative ion nanoelectrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (-)nanoESI-FTICR MS, investigation of complex biological mixtures consisting of sialylated or sulfated glycosphingolipids (GSL) expressing high heterogeneity in the ceramide portion is described. Different instrumental and solvent conditions were explored and optimized to promote efficient ionization, reduce the in-source fragmentation and consequently enhance the detection of intact molecular species from complex mixtures. Using the novel optimized (-)nanoESI-FTICR MS protocol, a reliable and detailed compositional fingerprint of the polysialylated ganglioside mixture isolated from human brain was obtained. Sustained off-resonance irradiation collision-induced dissociation mass spectrometry (SORI-CID MS2) was introduced for the first time for structural elucidation of polysialylated gangliosides. Under well-defined conditions, an informative fragmentation pattern of the trisialylated ganglioside GT1 was obtained. The compositional mapping of a complex mixture of sulfated glucuronic acid containing neolacto-series GSLs extracted from bovine Cauda equina provided hard evidence upon previously described components and new structures not identified before by any other analytical method. Negative ion nanoESI-FTICR MS at 9.4 T is shown here to represent a valuable method in glycolipidomics, allowing a high resolution and mass accuracy detection of major and minor GSL glycoforms and identification of known and novel biologically relevant structures.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
0108120

Ustanove
Medicinski fakultet, Zagreb

Autor s matičnim brojem:
Željka Vukelić, (241834)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • MEDLINE


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